Apr 12, 2021 · Optogenetic tools such as channelrhodopsin-2 (ChR2) enable the manipulation and mapping of neural circuits. However, ChR2 variants selectively transported down a neuron’s long-range axonal...

In this study, we generated an efficient optogenetic model of forebrain organoids with homogeneous and stable expression of ChR2 by selectively introducing ChR2 into the AAVS1 locus of hPSCs.

Viral vectors driving ChR2 expression can be delivered directly into specific brain regions with robust transduction efficacy and limited tissue damage. Adeno-associated viruses (AAVs) provide extensive spatial spread and high expression levels 12, 24.

Jun 9, 2024 · In this study, we generated an efficient optogenetic model of forebrain organoids with homogeneous and stable expression of ChR2 by selectively introducing ChR2 into the AAVS1 locus of hPSCs.

May 5, 2013 · Channel rhodopsin (ChR2) is expressed in neurons. When ChR2 labeling (A) is combined with immunocytochemistry for the neuronal markers MAP2 and TUJ1 (B), double labeling of neurons can be observed in the dorsal cochlear nucleus (C).

Nov 10, 2023 · For this purpose, we expressed the blue-shifted ChR2, oChIEF, employing a widely used AAV vector delivery strategy. We found that the volume of the injected virus has a minimal impact on the efficiency of optically-evoked postsynaptic population responses.

Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation

Apr 7, 2025 · To specifically and temporally activate RN excitatory neurons, we employed the optogenetic method as previously described [6]. In a cohort of adult WT mice, we injected adeno-associated virus (AAV) expressing channel rhodopsin 2 (ChR2) [20] under the CaMKII promoter, termed as AAV-CaMKII-ChR2, into the RN bilaterally.

Jul 2, 2015 · To permit neuron subtype specific gene expression in wildtype animals, we developed a combinatorial AAV targeting system that drives, in combination, subtype specific Cre-recombinase expression with a strong but non-specific Cre-conditional transgene.

Ready-to-use AAV1 particles produced from pAAV-mDlx-ChR2-mCherry-Fishell-3 (#83898). In addition to the viral particles, you will also receive purified pAAV-mDlx-ChR2-mCherry-Fishell-3 plasmid DNA.