Aug 29, 2018 · The regulation of TMEM173 mRNA was sufficient to affect STING protein levels as A549 cells lacking Nrf2, following CRISPR/Cas9 gene editing or treatment with two different Nrf2 targeting siRNAs...

GPR162 promotes DNA damage response in STING-dependent pathway. a DNA was identified using the PicoGreen fluorescence dye that specifically binds dsDNA in A549 cells treated with or without IR,...

Mar 31, 2021 · Deficiencies in DNA repair and DNA degrading nucleases lead to accumulation of cytosolic DNA. cGAS is a critical DNA sensor for the detection of cytosolic DNA and subsequent activation of the...

Apr 3, 2020 · A549 epithelial cells, commonly used for RNA-sensing studies, failed to respond to DNA because they lacked STING expression, and ectopic STING expression restored a cGAS-dependent DNA response in these cells.

Feb 28, 2024 · A colony formation assay demonstrated substantial growth arrest in A549 cells with induced STING expression (Fig. 4J), further confirmed by cell counting assay and 5-ethynyl-2-deoxyuridine (EdU) staining (Fig. 4K and fig. S4H).

IFN-γ-induced DNA damage activated the STING pathway. The DNA sensors cGAS and IFI16 are involved in STING activation induced by IFN-γ. Our results suggest that IFN-γ can shape the tumor immune microenvironment and exert antitumor effects …

Jan 19, 2023 · TP53 promotes the degradation of the cytosolic DNA exonuclease TREX1, resulting in cytoplasmic DNA accumulation and activation of the cGAS/STING pathway. The absence of cGAS or STING compromises p53’s tumor suppressor activity.

Jun 15, 2023 · This drug stress-induced STING downstream signaling activation was not confined to targeted therapies, as evidenced by an activation of STING signaling in A549 lung cancer cells underwent cisplatin treatment (Figure 4 H), extending to chemotherapy situations.

Stimulator of IFN genes (STING, also known as TMEM173, MITA, ERIS, and MPYS) is a cytoplasmic pattern recognition receptor shown to be an important transducer of Type I interferon (IFN) production triggered by the accumulation of double stranded DNA …

Stimulation of A549 cells with dsDNA led to significant induction of IFNb promoter activity in cocultured HEK293T cells expressing STING, but not in co-cultured HEK293T ...