In our study, we examined the role of ezrin in actin filament reorganization and cell meta-stasis during TGF-β1-induced alveolar EMT. Suppressing ezrin expression limited morphological changes and actin filament remodeling, decreased cell migration and invasion during EMT.

May 12, 2022 · In this study, we aimed to investigate the potential role of the rho-associated coiled-coil containing protein kinase (ROCK) pathway in LPS-induced ezrin phosphorylation and cytokine production in pulmonary alveolar epithelial cells. Cultured A549 and HPAEpiC cells were treated with LPS.

Mar 19, 2015 · Here, we explored the molecular mechanism by which ouabain exerts anticancer effects in human lung adenocarcinoma. Employing proteomic techniques, we found 7 proteins downregulated by ouabain in A549 including p-ezrin, a protein associated with pulmonary cancer metastasis in a dose-dependent manner.

Apr 13, 2019 · To summarize, the Cys 117 mutation of ezrin decreased basal ezrin tension in A549 cells, and inhibited the increase in ezrin tension after CM stimulation, suggesting the attenuated function of ezrin as a mechanical transducer.

Western blot analysis of the pool of active ERM proteins in A549-Epac1 cells revealed that siRNA against Ezrin and Moesin had the biggest effect on depletion of the phospho-ERM levels (Fig. 3F).

Feb 1, 2016 · To evaluate the effect of ezrin on EGFR phosphorylation, we silenced ezrin expression in A549 and H292 by small interfering RNAs. Cells were first serum starved for 24 hours and then treated with EGF for 5 minutes.

In this study, we aimed to investigate the potential role of the rho-associated coiled-coil containing protein kinase (ROCK) pathway in LPS-induced ezrin phosphorylation and cytokine production in pulmonary alveolar epithe-lial cells. Cultured A549 and HPAEpiC cells were treated with LPS.

Jun 1, 2011 · Knockdown of Ezrin, but not Radixin and Moesin, inhibits Epac-induced cell spreading. A549-Epac1 cells treated with siRNA against (A) Ezrin (siEzrin), (B) Radixin (siRadixin) or (C) Moesin (siMoesin) were replated and allowed to spread on fibronectin for 3 hours with 100 μM 007 or without 007 (mock). Focal adhesions and the actin cytoskeleton ...

In our study, we examined the role of ezrin in actin filament reorganization and cell meta stasis during TGF-β1-induced alveolar EMT. Suppressing ezrin expression limited morphological changes and actin filament remodeling, decreased cell migration and invasion during EMT.

These results demonstrated that ezrin is involved in regulating cell migration and invasion during EMT, further confirming ezrin is required for TGF-β1-induced EMT in A549 cells. Figure 4 Suppression of ezrin expression during EMT decreases cell migration and invasion.