Jan 18, 2015 · 5xFAD mice develop motor impairments, beginning at about 9 months of age (O’Leary et al., 2018; O’Leary et al., 2018). On the Rotarod, transgenic mice fall off more quickly than controls, and improve little across trials. 5xFAD mice also fall more quickly when tested on a balance beam, and in wire- and grid-suspension tests.

Jan 30, 2025 · In another study, which included mice of both sexes tested at 4, 8, 12 and 18 months, a genotype-dependent difference was seen only in the 8-month group (Forner et al., 2021). 5xFAD mice also spent more time than non-transgenic mice in the open arms of the elevated plus maze—generally thought to reflect reduced anxiety in this apparatus.

Jun 9, 2017 · APOE4 Targeted Replacement mice are available through Taconic, Stock# 1549-F or 1549-M. The E4FAD, E3FAD, and E2FAD mouse models are crosses between the widely used 5xFAD mice (Tg6799 line) and the APOE4, APOE3, and APOE2 Targeted Replacement mice, respectively. These models were created to study the role of the three human isoforms of …

Apr 6, 2018 · Humanized TREM2 mice were crossed with 5XFAD mice to obtain 5XFAD expressing the R47H variant (R47H + mTrem2 −/− 5XFAD, or R47H-KO-5XFAD) or the common variant (CV + mTrem2 −/− 5XFAD, or CV-KO-5XFAD). Additionally, mTrem2 −/− 5XFAD (KO-5XFAD) were available for comparison. Thus far, 8.5-month-old mice have been studied. …

Apr 6, 2018 · There was a modest decrease in PBS-soluble Aβ in hippocampus in CV-KO-5XFAD compared with KO-5XFAD mice. The neurodegeneration-associated microglial activation markers Cst7, Spp1, and Gpnmb were elevated in CV-KO-5XFAD compared with KO-5XFAD. CV-KO-5XFAD mice have also been compared with 5XFAD mice carrying the R47H variant of human …

Sep 19, 2024 · First author Claire Butler and colleagues wanted to know how this variant would affect amyloid plaques, so they initially crossed the V1613M strain to 5xFAD mice. The offspring reduced plaque load, dystrophic neurites, and plasma NfL. This was unexpected, Green said, because ABCA7 deficiency increases plaques.

Nov 27, 2020 · These data indicate that Aβ is the cause of neuronal degeneration and death in 5XFAD mice, rather than an artifact resulting from mutant APP and PS1 overexpression. Our work strongly suggests that Aβ is ultimately responsible for neuron loss in AD, a conclusion that has met with a fair amount of debate and controversy in the past.

Mar 21, 2024 · Curiously, transcriptomes of TREM1-deficient 5xFAD microglia were no different from control 5xFAD cells, both taking on a disease-associated microglia signature (Jun 2017 news). This made sense to Andreasson, since less than 5 percent of 5xFAD microglia expressed TREM1, just as in wild-type mice.

Jan 18, 2019 · 5xFAD mice were first crossed with Bace1 fl/fl mice in which exon 2 of the mouse Bace1 gene is flanked by loxP sites (Hu et al., 2018) to generate 5xFAD mice homozygous for a floxed Bace1 gene (BACE1 fl/fl/5xFAD). Bace1 fl/fl mice were also crossed with UBC-Cre-ERT2 mice (The Jackson Laboratory, Stock# 007001) to obtain BACE1 fl/fl/UbcCreER mice.

May 4, 2018 · The number of plaque-associated microglia was reduced in TREM2-BAC X 5xFAD (5xFAD/TREM2) mice compared with 5xFAD mice at 7 months of age. Microglial morphology also differed between genotypes: While plaque-associated microglia in 5xFAD cortex have an amoeboid shape, microglia in 5xFAD/TREM2 cortex have more elongated, ramified processes.