Combinatorial quantification of 5mC and 5hmC at individual CpG …
Apr 26, 2024 · Inheritance of 5-methylcytosine from one cell generation to the next by DNA methyltransferase 1 (DNMT1) plays a key role in regulating cellular identity.
DNMT1 regulates the timing of DNA methylation by DNMT3 in an ... - PubMed
Jan 5, 2022 · DNA methylation (DNAme; 5-methylcytosine, 5mC) plays an essential role in mammalian development, and the 5mC profile is regulated by a balance of opposing enzymatic activities: DNA methyltransferases (DNMTs) and Ten-eleven translocation dioxygenases (TETs).
Fine-tuning of gene expression through the Mettl3-Mettl14-Dnmt1 …
Feb 20, 2025 · Beyond its role as RNA m6A writer, METTL3-METTL14 can mediate 5mC deposition on DNA by recruiting the DNA methyltransferase DNMT1 to chromatin. The two marks co-orchestrate the expression of key genes in differentiating ESC, allowing proper exit …
DNMT1 regulates the timing of DNA methylation by DNMT3 in an …
DNA methylation (DNAme; 5-methylcytosine, 5mC) plays an essential role in mammalian development, and the 5mC profile is regulated by a balance of opposing enzymatic activities: DNA methyltransferases (DNMTs) and Ten-eleven translocation dioxygenases (TETs).
De novo DNA methylation drives 5hmC accumulation in mouse zygotes - Nature
Jan 11, 2016 · Instead, 5hmC accumulation is dependent on the activity of zygotic Dnmt3a and Dnmt1, documenting a role for Tet3-driven hydroxylation in targeting de novo methylation activities present in the...
Base editing-mediated one-step inactivation of the - Nature
May 22, 2023 · As expected, Dnmt1 or Dnmt3a/3b knockout embryos significantly decreased 5mC and 5hmC signals, but demethylation of imprinting regions occurred only at Dnmt1-KO embryos (Fig. 3d;...
DNMT1 regulates the timing of DNA methylation by DNMT3 in an …
Jan 5, 2022 · DNA methylation (DNAme; 5-methylcytosine, 5mC) plays an essential role in mammalian development, and the 5mC profile is regulated by a balance of opposing enzymatic activities: DNA methyltransferases (DNMTs) and Ten-eleven translocation dioxygenases (TETs).
DNA 5-Methylcytosine-Specific Amplification and Sequencing
Here, we present a 5mC-specific whole-genome amplification method (5mC-WGA), with which we achieved 5mC retention during DNA amplification from limited input down to 10 pg scale with limited interference from 5hmC signals, providing DNA …
5-methylcytosine turnover: Mechanisms and therapeutic …
In mammals, DNA methyltransferases (DNMTs) are responsible for the addition of a methyl group to the C5 position of cytosines to form 5-methylcytosines (5 mC). DNMT3A and DNMT3B drive de novo methylation during development, while DNMT1 is involved in maintaining DNA methylation by copying methylation patterns onto the newly replicated DNA strand.
Cell: Fine-tuning of gene expression through the Mettl3-Mettl14-Dnmt1 …
Jan 17, 2025 · This study shows that METTL3-METTL14 recruits the DNA methyltransferase DNMT1 to chromatin for gene-body methylation. We identify a set of genes whose expression is fine-tuned by both gene-body 5mC, which promotes transcription, and m 6 …