The 5′-end (pronounced "five prime end") designates the end of the DNA or RNA strand that has the fifth carbon in the sugar-ring of the deoxyribose or ribose at its terminus.

The below protocol is for 5' end labeling RNA with PNK. The labeled RNA can be used as a probe or for RNA structure assessment, protein footprinting, and boundary determination experiments.

Jun 4, 2018 · A direct comparison of 5′-end RNA-seq methods reveals strong performance by CAGE, and identifies differential transcriptional start site usage among brain-related samples.

Here, we provide a comprehensive overview of the complexity of RNA cap structures and 5′-triphosphate RNAs, highlighting their roles in transcript identity, immune surveillance, and disease. Keywords: 5′ cap, m7G, IFIT, RIG-I, capping, …

Feb 16, 2014 · The 5' and 3' mean "five prime" and "three prime", which indicate the carbon numbers in the DNA's sugar backbone. The 5' carbon has a phosphate group attached to it …

The function and fate of cellular RNAs are often governed by the phosphorylation state at the 5′ end or the identity of whatever cap may be present there. Here we describe methods for examining these important 5′-terminal features on any cellular or ...

Mar 18, 2024 · RNA caps are deposited at the 5' end of RNA polymerase II transcripts. This modification regulates several steps of gene expression, in addition to marking transcripts as self to enable the innate immune system to distinguish them from uncapped foreign RNAs, including those derived from viruses.

Jul 21, 2016 · The chemical nature of the 5′ end of RNA is a key determinant of RNA stability, processing, localization and translation efficiency, and has been proposed to provide a layer of ‘epitranscriptomic’ gene regulation.

Here, we directly compare the performance of six such methods – testing five with cellular RNA as well as a novel spike-in RNA assay that helps address interpretation challenges that arise from uncertainties in annotation or RNA processing.

Feb 15, 2019 · The function and fate of cellular RNAs are often governed by the phosphorylation state at the 5' end or the identity of whatever cap may be present there. Here we describe methods for examining these important 5'-terminal features on any cellular or synthetic RNA of interest that can be detected by …