50S is the larger subunit of the 70S ribosome of prokaryotes, i.e. bacteria and archaea. It is the site of inhibition for antibiotics such as macrolides, chloramphenicol, clindamycin, and the pleuromutilins. It includes the 5S ribosomal RNA and 23S ribosomal RNA.

Oct 22, 2024 · The 50S ribosomal subunit is a marvel of molecular architecture, composed of ribosomal RNA (rRNA) and proteins that interlock to form a functional unit. This subunit is primarily made up of two rRNA molecules, the 23S and 5S …

Aug 10, 2015 · Mechanistic studies revealed that RlmE-mediated Um2552 formation promotes interdomain interactions of 23S rRNA, in concert with the incorporation of L36, triggering late steps of 50S subunit assembly. RlmE and Um2552 are conserved in other organisms, including human, indicating the functional importance of this process in ribosome biogenesis.

Feb 22, 2002 · The 50S subunit binds the acceptor arms of tRNA and catalyzes peptide bond formation between the incoming amino acid on A-site tRNA and the nascent peptide chain attached to the P-site tRNA. Both subunits are involved in translocation, in which the tRNAs and mRNA move precisely through the ribosome, one codon at a time.

The 50S ribosomal subunit is composed of 23S rRNA (2900 nts), 5S rRNA (120 nts) and more than 30 ribosomal proteins and is responsible for catalyzing peptide bond formation. You might find these chapters and articles relevant to this topic.

Jun 7, 2018 · Our structures monitor the formation and stabilization of structural elements in a nascent particle in unprecedented detail and identify the maturation of the rRNA-based peptidyl transferase center as the final critical step along the 50S assembly pathway.

Jun 7, 2018 · Our structures monitor the formation and stabilization of structural elements in a nascent particle in unprecedented detail and identify the maturation of the rRNA-based peptidyl transferase center as the final critical step along the 50S assembly pathway.

Using a 2.4 Å resolution, experimentally phased, electron density map, we have pro-duced an atomic structure of the H. maris-mortui 50S ribosomal. The model includes 2711 of the 2923 nucleotides of 23S ribosom-al RNA (rRNA), all 122 nucleotides of its 5S rRNA, and structures for the 27 proteins that are well ordered in the subunit.

Oct 2, 2000 · Ribosomal proteins L2, L3 and L4, together with the 23S RNA, are the main candidates for catalyzing peptide bond formation on the 50S subunit. That L2 is evolutionarily highly conserved led us to perform a thorough functional analysis with reconstituted 50S particles either lacking L2 or harboring a …

Assembly map of the large subunit (50S) of Escherichia coli ribosomes. Stoichiometric amounts of ribosomal proteins and RNA derived from the 50S subunit reconstitute to fully active particles under the conditions of a two-step incubation procedure.