Thermo Scientific 5-Fluoroorotic Acid (5-fluorouracil-6-carboxylic acid monohydrate; 5-FOA) is used in yeast molecular genetics to detect expression of the URA3 gene, which encodes …

5-Fluoroorotic acid (5FOA) is a fluorinated derivative of the pyrimidine precursor orotic acid. It is used in yeast genetics to select for the absence of the URA3 gene, which encodes the …

Using 5-Fluoroorotic Acid (5-FOA) for the counter-selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and …

Positive selection is carried out by auxotrophic complementation of ura3 mutations, whereas highly discriminating negative selection is based on the specific inhibitor 5-fluoro-orotic acid (5 …

5-Fluoroorotic Acid (5-FOA) Plates. 0.67% yeast nitrogen base. 0.2% SC–Ura drop-out mix. 2% glucose. 50 µg/mL uracil. 0.1% 5-FOA. Combine the ingredients above, and add distilled water …

5-Fluoroorotic acid | C5H3FN2O4 | CID 69711 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity …

5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of …

Jan 1, 1987 · The 5-fluoroorotic acid (5-FOA) selection is extremely useful for a number of genetic and molecular biological manipulations requiring the detection of rare ura3- cells.

A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions. 5-FOA is an extremely …

ribosomal RNA in rat liver cells. 15 FOA is a noncompetitive inhibitor of dihydroorotase. 16 FOA (50% inhibition, 6 nM) was a selective inhibitor of malarial cells of Plasmodium falciparum in …