Mar 27, 2023 · For necroptosis induction, the starved and TNF-α-primed (4 h) 4T1 tumor cells were treated with pan-caspase inhibitor QVD-OPh (10 μM) before Navticlax (10 μM) addition.

Mar 9, 2015 · Studies of tumor models using syngeneic transplantation have advanced our understanding of tumor immunity, including both immune surveillance and evasion. Murine mammary carcinoma 4T1 cells secrete...

A gene array study confirmed that TNF-α dependent and independent apoptotic pathways are down regulated in 4T1 cells. B. Cell viability of TNF-α treated 4T1 and 67NR cells was assessed by ...

Nov 15, 2020 · We report that mouse 4T1 breast cancer cell-derived exosomes enhanced TAM expression of IL-1β, IL-6, and TNF-α and that inhibition of 4T1-cell exosome secretion through short hairpin RNA-mediated Rab27a/b depletion repressed tumor growth and metastasis and markedly downregulated IL-1β, IL-6, and TNF-α in a 4T1 breast tumor model.

Using 4T1 cells and human TNBC cell lines, they demonstrated that TNFα induces COP9 signalosome 5 (C5N5), a protein that inhibits PD-L1 ubiquitination, through NF-κB activation. Then, PD-L1 protein increases its stability in tumor and …

Apr 20, 2020 · In mouse breast cancer (4T1) subcutaneous model, rAd.Light produced significant anti-tumor response via inducing apoptosis of tumor cells, increasing infiltration of T lymphocytes and...

Jul 6, 2022 · Applying the traditional TNF-α antibodies that neutralize TNF-α activity, however, only exerts modest anti-tumor efficacy in clinical studies. Here, we develop an innovative approach to target TNF-α that is distinct from the neutralization mechanism.

LyeTx I-b is a synthetic peptide derived from Lycosa erythrognatha spider venom that previously showed antibiotic activity in vitro and in vivo. This study focused on the effects of LyeTxI-b on a 4T1 mouse mammary carcinoma model.

Nov 10, 2020 · Exosomes released by tumor cells play critical roles in tumor progression, immune cell suppression, and cancer metastasis. The aim of the present study was to investigate whether the exosomes released by EL4 cells carry a functional TNF-related apoptosis-inducing ligand (TRAIL) molecule.

Feb 1, 2014 · Consistently, our in vivo study showed that ectopic expression of uncleavable tmTNF-α mutant by 4T1 cells significantly promoted tumor progression and angiogenesis, accompanied with more accumulation of MDSCs and regulatory T cells in the tumor site, increased production of NO, IL-10, and TGF-β, as well as poor lymphocyte infiltration.