Jan 4, 2023 · To this end, we show that, using solvatochromic study and molecular dynamics simulations, the frequency of maximum emission intensity of the fluorophore of 4-cyanotryptophan (4CN-Trp), 3-methyl-1 H -indole-4-carbonitrile, exhibits a linear dependence on the local EF.

Jan 1, 2020 · In this chapter, we describe the synthesis and spectroscopic utility of two indole-based fluorophores, 4-cynaotryprophan (4CN-Trp) and 4-cyanoindole-2′-deoxyribonucleoside (4CNI-NS), with a focus on 4CN-Trp.

The steady state and time-resolved fluorescence and infrared (IR) properties of 4- and 5-cyanotryptophan (CNTrp) are investigated and compared, and the tryptophan (Trp) analogs are found to be very attractive to study structural and dynamic properties of proteins.

To show that Trp is the only amino acid that can significantly quench the fluorescence of 4CN-Trp, we studied the fluorescence property of a series of peptides with the sequence of 4CN-Trp-X (when X is a polar/charged residue) or 4CN-Trp-X-AAKKK (when X is a hydrophobic residue).

Herein, we show that a Trp analog, 4-cyanotryptophan (4CN-Trp), meets these requirements and has great potential to expand biological fluorescence spectroscopy and microscopy into additional territory.

May 22, 2023 · Combining transient mid-infrared (TRIR) and transient UV/Vis absorption (TA) measurements, we have resolved the photo-induced redox cycle of the W 5CN –W motif by capturing and quantifying the elusive charge-separation species W 5CN·− and W ·+.

Herein, we aim to describe a simple, high-yielding, and cost-effective synthetic route for L-4CN-Trp and Fmoc-L-4CN-Trp and demonstrate the utility of L-4CN-Trp fluorescence in the study of peptide-membrane interactions, under both in vitro and in vivo conditions.

Feb 19, 2021 · The red-shifted emission of water-exposed W 4CN residues was leveraged to investigate the solvation state of tryptophan (Trp) residues within the influenza M2 proton channel.

In this chapter, we describe the synthesis and spectroscopic utility of two indole-based fluorophores, 4-cynaotryprophan (4CN-Trp) and 4-cyanoindole-2'-deoxyribonucleoside (4CNI-NS), with a focus on 4CN-Trp.

Jan 1, 2020 · Fourth, 4CN-Trp (or 4CNI) is the most fluorescent among all cyanotryptophans (Fig. 3) with a large fluorescence QY (> 0.8), a long fluorescence lifetime (ca. 13.7 ns in water), good photostability (Fig. 4A), and an emission spectrum in the visible range.