Jan 1, 2020 · Here, we present an improved step-by-step protocol for sample preparation and the generation of 4C-seq sequencing libraries, including an optimized PCR and 4C template …

Jan 1, 2020 · 4C-seq identifies chromatin loops, contact domains and inter-domain contacts. A detailed protocol for preparation of 4C-seq template is provided. A pipeline is provided which …

4C, also called 4C-seq, is a method similar to 3C and is sometimes called circular 3C. It allows the unbiased detection of all genomic regions that interact with a particular region of interest. …

Here we aim to focus on the current state of the 4C-Seq method and the limitations and challenges of the associated computational analysis. Analysis of 4C-Seq data can be …

In summary, our package provides the tools to analyze 4C sequencing data and integrate the results with other genomic features. Its use will help to further investigate and understand the …

Chromosome conformation capture combined with high-throughput sequencing (4C-seq) is a powerful tool to map genomic DNA regions that communicate with a specific locus of interest …

Aug 21, 2020 · Among the method derivatives, 4C-seq (circular chromosome conformation capture, coupled to high-throughput sequencing) is a versatile, cost-effective means of …

Here, we discuss in detail the design, application, and data analysis of 4C-seq experiments. Based on many hundreds of different 4C-seq experiments, we define criteria to assess data …

Jul 1, 2019 · Here, we present an improved step-by-step protocol for sample preparation and the generation of 4C-seq sequencing libraries, including an optimized PCR and 4C template …

Feb 1, 2022 · Here we describe a 4C-seq protocol that has been optimized for primary adherent cells, for which the first digestion step is inefficient using standard 4C-seq protocols. It can, …