4-Hydroxynonenal, or 4-hydroxy-2E-nonenal or 4-hydroxy-2-nonenal or 4-HNE or HNE, (C 9 H 16 O 2), is an α,β-unsaturated hydroxyalkenal that is produced by lipid peroxidation in cells. 4-HNE is the primary α,β-unsaturated hydroxyalkenal formed in this process. It is a colorless oil.

4-HNE is considered one of the major mediators of oxidative stress in cells and tissues, that collectively lead to cell senescence by affecting the expression of various senescence-related signaling pathways (such as NF-κB, Nrf2, Akt/PKB, and mTOR), as shown in Figure 2.

Mitogenic responses of vascular smooth muscle cells to lipid peroxidation-derived aldehyde 4-hydroxy-trans-2-nonenal (HNE): role of aldose reductase-catalyzed reduction of the HNE-glutathione conjugates in regulating cell growth.

Oct 4, 2013 · 4-hydroxy-2-nonenal (HNE), a secondary product of lipoperoxidation, can form protein adducts and modifies cell signaling. Because of its chemical reactivity, HNE can exert pleiotropic...

Our results indicate that 4-HNE decreases human SAEC viability with an increase in cleaved caspase-3 activity. 4-HNE augmented the production of mitochondrial ROS, followed by a reduction in mitochondrial oxygen consumption.

Jul 24, 2017 · Oxidative stress (OS)-induced lipid peroxidation and the consequent generation of highly electrophilic aldehydes, such as 4-hydroxynonenal (4-HNE), represents a potential mechanism by which ROS...

Sep 30, 2015 · This review on recent research advances of the lipid peroxidation product 4-hydroxy-nonenal (HNE) has four major topics: I. the formation of HNE in various organs and tissues, II. the diverse biochemical reactions with Michael adduct formation as the most prominent one, III. the endogenous targets of HNE, primarily peptides and proteins (here ...

Oct 1, 2017 · 4-Hydroxynonenal (HNE) forms protein adducts during oxidative stress. HNE modification occurs at several amino acids side chains in a variety of proteins. Reaction with HNE affects function and fate of modified proteins. HNE-modified proteins might act as biomarkers.

Oct 10, 2019 · Here we present a reproducible, facile, and economically conservative protocol for quantifying HNE protein adducts. The key to this protocol is to generate HNE-adduct standards by incubating bovine serum albumin (BSA) with HNE. These standards are then adsorbed to immunsorbent plastic in a multi-well plate format alongside biological samples.

4-Hydroxynonenal (HNE), an electrophilic end-product deriving from lipid peroxidation, undergoes a heterogeneous set of biotransformations including enzymatic and non-enzymatic reactions.