3T3-L1 is a sub clonal cell line derived from the original 3T3 Swiss albino cell line of 1962. The 3T3 original cell line was isolated from a mouse embryo and propagated for this specific line of 3T3 cells is used to study adipose tissue -related diseases and dysfunctions.

3T3-L1 is a fibroblast that was isolated from the embryo of a mouse. This cell line can be used to study the basic cellular mechanisms associated with diabetes, obesity, and related disorders.

Jun 1, 2012 · In this note, we present a detailed procedure for highly effective and reproducible 3T3-L1 cell differentiation. Due to their potential to differentiate from fibroblasts to adipocytes, 3T3-L1 cells are widely used for studying adipogenesis and the biochemistry of adipocytes.

We find that newly thawed, contact inhibited 3T3-L1 cells are adipogenic, but they lose adipogenic potential after being maintained in culture at confluence and losing contact inhibition. Thus, the differentiation potential of 3T3-L1 is dependent on contact inhibition.

Here, we show that NOX3 expression is induced in differentiated 3T3-L1 adipocytes upon treatment with proinflammatory cytokines. Superoxide production increased concurrently with NOX3 protein expression in cytokine-treated adipocytes, which was inhibited by the NOX inhibitor diphenyleneiodonium (DPI).

Differentiation of multi-potential 3T3-L1 fibroblasts into lipid laden adipocyte-like cells is one of the most common in vitro models used in the study of adipocyte biology.

Jan 16, 2025 · The 3T3-L1 cell line, a mouse embryonic fibroblast cell line, was first isolated by Green and Kehinde in 1974. Due to its ability to differentiate into adipocyte-like cells in vitro, it has been widely used in the study of metabolic diseases such as obesity and diabetes.

The 3T3-L1 cell line is commonly used to study adipocyte biology, adipocyte cell differentiation, and related cellular and molecular mechanisms. The differentiation of 3T3-L1 cells into adipocytes is closely mimics the in vivo differentiation pathway of adipocytes.

Robust and well-characterized, 3T3-L1 pre-adipocytes differentiate and form fat pads indistinguishable from normal adipose tissue when injected into mice. In this protocol, differentiation is achieved in vitro through the sequential application of adipogenic factors to 3TC-L1 cells over a period of several days in culture. Following

The 3T3-L1 cell-line is a widely used model system for the analysis of adipocyte differentiation. The characteristic of this cell line is its ability to differentiate into mature adipocytes upon stimulation with insulin, dexamethasone and methylisobutylxantine when maintained in …