3T3 cells are several cell lines of mouse embryonic fibroblasts. The original 3T3 cell line (3T3-Swiss albino) was established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green .

NIH/3T3 is a fibroblast cell line that was isolated from a mouse NIH/Swiss embryo. This cell line is highly sensitive to sarcoma virus focus formation and leukemia virus propagation and has proven to be very useful in DNA transfection studies.

The embryonic mouse fibroblast cell line NIH3T3 is widely used in life science research, including the study of cell cycle control and primary cilia. Fibroblasts are the most important cell type in connective tissue, as they produce components of ...

With an average diameter of about 18 μm, NIH-3T3 cells offer a versatile model for in-depth cell biology studies, ranging from tissue repair mechanisms to the intricate pathways of cell cycle regulation.

In the case of mouse embryo cells, for example, this frequently occurs, and a well-known cell line derived from mouse embryo cells in this way is called the 3T3 cell line. Cell lines are sometimes referred to as being “immortal” because of their ability to proliferate indefinitely in culture.

Cryoprotectant Medium – Complete growth media + 5% (v/v) DMSO (1*106 cells / mL) Initiating Culture 1. Place a T-75 with complete growth medium into an incubator for at least 15 mins to allow the medium to reach its normal pH. 2. Thaw the vial by gentle agitation in a 37 C water bath (should take less than two mins) a.

Nov 13, 2015 · Tubulin, Actin, and DNA Distribution in 3T3 Cells - A culture of 3T3 cells was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to the cyanine dye, Cy3. In addition, the cells were simultaneously probed for DNA with the ultraviolet-absorbing probe DAPI, and ...

The murine cell line NIH 3T3 has been used as a model system in a multitude of different studies since its first description in 1963. It was established from Mus musculus f. domenstica (“Swiss mouse”) embryo fibroblasts.

3T3-L1 is a fibroblast that was isolated from the embryo of a mouse. This cell line can be used to study the basic cellular mechanisms associated with diabetes, obesity, and related disorders.

DT cells (provided by Dr. Yoon Sang Cho-Chung), which had been transformed with k-ras and are malignant k-ras expressing cells, were derived from NIH 3T3 cells (Noda et al., 1983) and used for stable transfection with a siTR1/siGPx1 double knockdown vector (Xu et al., 2009) as described below. C57BL/6 mice were used for a tumor formation assay ...