Here, we introduce an N terminomics toolbox that combines selective N-termi-nal biotinylation using 2-pyridinecarboxaldehyde (2PCA) reagents with chemically cleavable linkers to enable …

The imidazolidinone formed by 2PCA conjugation imparts a unique residual mass (C 6 H 3 N + 89.0265 Da) that can be used to diagnostically assign low mass ions that are similar to typical …

Mar 21, 2024 · Here, we introduce an N terminomics toolbox that combines selective N-terminal biotinylation using 2-pyridinecarboxaldehyde (2PCA) reagents with chemically cleavable …

Mar 30, 2015 · We present a versatile method for the selective modification of protein N termini that does not require any genetic engineering of the protein target. This reaction is …

Feb 13, 2023 · By incorporating a commercially available alkyne-modified 2PCA in combination with Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC), our strategy eliminates the need for …

Feb 13, 2023 · Here, we introduce an expanded N terminomics toolbox that is based on 2-pyridinecarboxaldehyde (2PCA) reagents. These tools enable efficient enrichment of protein N …

In this section, we experimentally evaluate our proposed (2D)2PCA method with PCA, 2DPCA and alternative 2DPCA, on two well-known face databases: ORL and FERET [5].

Feb 13, 2023 · By incorporating a commercially available alkyne-modified 2PCA in combination with Cu (I)-catalyzed azide-alkyne cycloaddition (CuAAC), our strategy eliminates the need for …

This work describes the development of a site-specific N-terminal modification strategy using 2-pyridinecarboxaldehyde (2PCA) derivatives. The 2PCA reaction has been shown to effectively …

Aug 30, 2023 · They showed a specific reaction of the 2-pyridinecarboxaldehyde (2PCA) moiety with the N-terminus of peptides and proteins. The aim of this research was to combine this …