Sep 15, 2020 · Herein, we propose a powerful two-dimensional hybridization chain reaction (2D HCR) strategy for amplified sensing and imaging of intracellular mRNA. The basis is the design of ternary hairpin or dumbbell-structured DNA fuel strands.

In situ hybridization chain reaction (HCR) is a method to visualize specific mRNAs in diverse organisms by applying a HCR that is an isothermal enzyme-free nucleotide polymerization method using hairpin DNAs.

Hybridization chain reaction (HCR) provides a feasible solution for nucleic acid detection without target amplification. By highly specific sandwich hybridization, target RNA can be directly captured onto solid support and detected using HCR with fluorescent dyes.

Motivated by this knowledge, we have engineered a compact and clamped cascaded DNA circuit for specifically discriminating cancer cells from normal cells via the amplified multi-microRNA imaging strategy.

May 15, 2016 · R1 was the responses of the first dimension HCR and R2 was the responses of the 2D HCR. Measurements were performed at 25 °C. Here we report a proof-of-concept demonstration of a novel, HCR-based, direct molecular detection method using only ordinary oligonucleotides.

Aug 17, 2020 · Herein, we propose a powerful two-dimensional hybridization chain reaction (2D HCR) strategy for am-plified sensing and imaging of intracellular mRNA. The basis is the design of ternary...

Sep 1, 2017 · A powerful two-dimensional hybridization chain reaction (2D HCR) strategy for am-plified sensing and imaging of intracellular mRNA with high signal-to-noise ratio is proposed and MnO2 nanosheets/GSH-aided transportation and release association is successfully applied to adapt the 2D H CR nanosystem in live cells.

Aug 12, 2019 · To show that this new, branching HCR system offers a route for enzyme-free, label-free DNA detection, we demonstrate the multiplexed detection of a target sequence (as the initiator) in whole...

Confocal laser scanning microscopy images of mRNA based on 1D HCR without and with MnO 2 nanosheets.

Jul 17, 2017 · In a typical HCR, an analyte initiates the cross-opening of two DNA hairpins, yielding nicked double helices that are analogous to alternating copolymers. As an efficient amplification platform, HCR has been utilized for the sensitive detection of a wide variety of analytes, including nucleic acids, proteins, small molecules, and cells.