HEK-Blue™ TGF-β cells are designed to monitor human TGF-β-induced Smad stimulation or inhibition. This colorimetric bioassay can be used to screen activatory or inhibitory molecules, such as engineered cytokines and neutralizing antibodies, respectively.

Oct 9, 2006 · To determine whether Cripto affects TGF-β signaling, we first tested its ability to modulate TGF-β-dependent Smad2 phosphorylation. 293T cells were stably infected with lentivirus containing either Cripto or empty vector and then treated with a range of TGF-β1 doses.

We constructed a TGF-β bioassay which can detect both soluble and membrane-bound forms of TGF-β from T cells. For this bioassay, 293T cells were transduced with (caga) 12 Smad binding element-luciferase along with CD32 (Fc receptor) and CD86.

Aug 31, 2008 · To elucidate the molecular mechanisms for TGF-β-induced activation of TAK1, we performed in vivo ubiquitylation assays in human embryonic kidney (HEK) 293T cells transfected with TAK1 and...

HEK293/SBE-Luc2P is designed for monitoring the TGF-β/SMAD signaling pathway activity and used to screen activators or inhibitors of TGF-β/SMAD signaling pathway. Recombinant HEK293 SBE reporter stable cell line expresses luciferase in response to activation of TGFβ/SMAD signaling pathway.

Stable 293T cell line expressing exogenous luciferase gene under the control of TGFβ signaling pathway. Quantity: Two vials of frozen cells (over than 5×10 6 )

HEK-293T cells were transiently transfected with either the TGF-/activin-responsive CAGA reporter (A) or the BMP-responsive BRE reporter (B). Cells were incubated for 24 h in the absence (cont)...

It is a transmembrane protein highly expressed by activated Tregs and plays a key role in the activation of TGF - β, thereby promoting the immunosuppressive effect of Tregs. The overexpression of GARP in human cancers is associated with tolerance to TME and adverse clinical reactions to ICB, indicating that GARP blockade may improve cancer ...

It is a transmembrane protein highly expressed by activated Tregs and plays a key role in the activation of TGF - β, thereby promoting the immunosuppressive effect of Tregs.

We treated cells with 10 ng/ml TGF-b1 for 60 min to increase the phosphorylation level of Smad2/3 and found that Smad2/3 phosphorylation was upregulated in cells expressing IPP5 and IPP5 T34D ......