By expressing green fluorescent protein (GFP) as a reporter protein using the calcium phosphate transfection method and imaging a large population of cells, we found that a 5-min exposure of 10 % DMSO to HEK-293 cells, 4 h after transfection of the protein of interest, leads to ~1.6-fold increase in the expression yield without causing any ...

Transformed from adherence-enhanced HEK293 cells, the cell line expresses the enhanced GFP under CMV promoter. The cell line carries the Blasticidin resistance, see the integrated …

Aug 29, 2017 · We have demonstrated dual-color endogenous protein tagging with sfCherry2 11 and GFP 11, revealing that endoplasmic reticulum translocon complex Sec61B has reduced abundance in certain peripheral...

Dec 19, 2024 · GFP was visibly expressed between 8 and 24 h after 293T transfection by constructs to produce lentivirus for CAR T cells production, and GFP expression increased between 24 and 48 h after transfection, and between 48 and 72 h after transfection (Figure S1).

Mar 15, 2010 · We used a mammalian green fluorescent protein (GFP) expression system to express HPV-18E6 and HPV-6E6 fusion proteins in wild-type (wt) p53 cell lines, 293T and HEK293 cells, to investigate the traffic and location of E6s and p53.

293T‐GFP1‐10 and ‐GFP11 (1:1 ratio) were transfected with ACE2 and infected with SARS‐CoV‐2 at the indicated MOIs. Cell fusion was visualized by the GFP + area 18 h post‐infection.

Here, we evaluated five lentiviral vectors containing GFP gene and a combination of different promoters, HS4 insulator, or UCOE upon transducing the HEK 293T cells in terms of the stability and the efficiency of GFP expressing using fluorescent microscopy and flow cytometry.

Use GFP fluorescence for live cell imaging and FACS, or luciferase activity to quantitatively measure activity of the signaling pathway. Study NF-κB signaling in 293 TN cells

Jun 29, 2022 · dual‐labeled HEK-293T cell line Description: This product is a dual‐. abeled stable pool in the designated cell type. This stable cell line expre. ses firefly luciferase and eGFP simultaneously. This cell line can be used in vitro for cancer cell line research or in vivo to es. ablish orthotopic or subcutaneous tumor models. Tumor gr.

Mar 22, 2019 · Methylome data obtained from human embryonic kindey (HEK)-293T cells expressing a GFP (293T-GFP) or a truncated form of Arabidopsis DEMETER (DME) 5 …