I am growing the HEK293T cells in MEM currently and they are doing all right, but I wonder if I should switch to DMEM because it is their intended medium.

Seed HEK293T cells at 3.8x10 6 cells per plate in DMEM complete in 10 cm tissue culture plates. Incubate the cells at 37 °C, 5% CO 2 for ~20 h. For 10 mL of DMEM complete, add 10 µL of 25 mM chloroquine diphosphate. Dilute 18.9 µg of DNA into 500 µL of Opti-Pro SFM.

Use complete Dulbecco's modified Eagle's medium, supplemented with the following: In addition, 20 µg/mL ciprofloxacin can be used to prevent mycoplasma contamination. What's this? Copyright © 2025 by Cold Spring Harbor Laboratory Press. Terms of Service.

there is a morphological difference.. but what actually DMEM f12 does to cells? Hope the paper mentioned below will be of some help to you. they will grow just fine. Got a technical question? …

Recently, our group developed a 293T cell transient transfection-based cell adhesion assay to demonstrate interactions between highly sialylated B16F10 murine melanoma cells and sialic acid binding protein Siglec1 by using fluorescence microscopy (Singh and Choi, 2019).

ATCC recommends the following media for 293T cells: DMEM (ATCC 30-2002) + 10% heat inactivated FBS (ATCC 30-2020 (however, this product is NOT heat inactivated, just filtered)), and 2 mM L-glutamine (ATCC 30-2214).

Cell culture: 293T cells were grown in DMEM (invitrogen) supplemented with 10% FBS (Hyclone) at 37oC in 5% CO2 using 100mm culture dish. Subculture: Check the confluence of the cell using an inverted microscope. Prepare to sub-culture the cells when they reach ~80% confluence. Carefully aspirate old medium from the cells.

Feb 20, 2022 · Here we describe the optimized transfection of HEK 293T cells in both culture formats. DMEM was the best transfection medium for adherent HEK 293T cells, so we determined the kinetics of linear polyethyleneimine (LPEI) polyplex formation with plasmid DNA (pDNA) and subsequent cellular uptake.

HEK-293 T cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10 % fetal bovine serum (Atlanta Biologicals) and penicillin-streptomycin (50 units/mL and 50 μg/mL, respectively, final concentrations).

Medium 293T/HT1080 Dulbecco’s modified Eagle’s medium (DMEM) with high glucose (4500 mg/l), supplemented with: 10% (v/v) fetal bovine serum (FBS), 100 units/ml penicillin, and 100 μg/ml streptomycin.