Characteristics of FreeStyle™ 293-F cells: • Prepared from low-passage Master Cell Bank cultures derived from parental 293-F cells that were re-cloned by limiting dilution. The 293 clonal derived cultures are maintained in serum-free conditions for only 30 to 35 total passages.

Human Embryonic Kidney (HEK) cell line (e.g., Freestyle HEK 293F cells). These cells have been derived from the HEK 293 cell line, are adapted to grow in suspension cultures, reaching high ...

293-F cells are GIBCO brand cells. These are clonal isolates derived from transformed human embryonal kidney (HEK) cells. The 293-F strain is a fast-growing variant of the 293 cell line. They have been adapted to suspension culture in serum-free media, 293 SFM II.

FreestyleTM 293-F cells from Life Technologies are prone to clumping at higher densities and volumes. Polyethylenimine (PEI) (25 kDa linear PEI, Polysciences, Inc., cat. No. 23966) is prepared as a stock solution at a concentration of 1 mg/ml in a buffer containing 25mM HEPES and 150 mM NaCl (pH 7.5).

The FreeStyleTM 293-F cell line is derived from the 293 cell line (see “Parental cell line“ on page 6) and is intended for use with the FreeStyleTM MAX 293 Expression System (Cat. no. K9000-10) or the FreeStyleTM 293 Expression System (Cat. no. K9000-01).

Expi293F human cells are derived from the 293 cell line, and are a core component of the Expi293 Expression System. They are maintained in suspension culture and will grow to high density in Expi293 Expression Medium.

including adenoviral, lentiviral, and retroviral vectors. Their robust growth in suspension and ease of transfection make them ideal for use in transient transfection protocols, where they can produce h. gh yields of protein within a few days post-transfection. This characteristic is critical for ra.

Studies have demonstrated maximal virus production in human 293 cells expressing SV40 large T antigen (Naldini et al., 1996), making the 293FT Cell Line a particularly suitable host for generating lentiviral constructs using the ViraPowerTM Lentiviral Expression System available from Thermo Fisher Scientific (Cat. no. K4950-00 and K4960-00).

293F细胞是一类能够在无血清中高表达蛋白的野生型293细胞系。 293FT细胞则是在293F细胞系中插入pCMVSPORT6TAg.Neo 质粒的转化细胞系，能够快速增值，易转染，其能够用于慢病毒载体的生产。 293FTM细胞则是来源于 Flp-InTMT-RETM293细胞，该细胞系中转染了ecotropic receptor质粒及MAPPIT (mammalian protein-protein interaction trap)报告质粒，该细胞系主要用于蛋白互作的筛选。 293S细胞则指的被驯化成能够悬浮培养且能够耐受低钙离子培养条件 …

May 28, 2016 · In fact, HEK 293F cells are straightforward to grow, transfect with high efficiency and often produce significant yields of recombinant proteins. In this unit, we describe a method to express and purify milligram quantities of a human protein complex from HEK 293F cells grown in suspension transiently transfected with the appropriate plasmids ...