Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, are an immortalised cell line derived from HEK cells isolated from a female fetus in the 1970s. [1] [2] The HEK 293 cell line has been widely used in research for decades due to its reliable and fast growth and propensity for transfection.

293 [HEK-293] is a cell line exhibiting epithelial morphology that was isolated from the kidney of a human embryo. This cell line can be used in industrial biotechnology and toxicology research. It has applications in efficacy testing and viruscide testing.

Among the human cell lines, HEK293 is widely utilized due to its high transfectivity, rapid growth rate, and ability to grow in a serum-free, suspension culture. In this review, we discuss the use of HEK293 cells and its subtypes in the production of biotherapeutics.

The HEK293 cell line is a permanent line established from primary embryonic human kidney, which was transformed with sheared human adenovirus type 5 DNA. The adenoviral genes expressed in this cell line allow the cells to produce very high levels of recombinant proteins.

Jun 18, 2021 · Human Embryonic Kidney 293 (HEK 293) cells are one of the most frequently used cell lines for biotechnology and research studies. These cells are quite popular because of their distinct properties like fast-growing, robustness, and ease of maintenance and transfection.

The 293T cell line, originally referred as 293tsA1609neo, is a highly transfectable derivative of human embryonic kidney 293 cells, and contains the SV40 T-antigen. This cell line is competent to replicate vectors carrying the SV40 region of replication. It gives high titers when used to produce retroviruses.

Feb 6, 2023 · HEK293 cells are Human Embryonic Kidney cells, originally isolated and grown by Dutch biologist Alex Van der Eb in the early 1970s. They were transfected with sheared adenovirus 5 (Ad5) DNA by Frank Graham, a postdoc in Van der Eb’s lab.

HEK-293 cells were generated in 1973 by transfection of normal healthy human embryonic kidney (HEK) cells with adenovirus 5 DNA by Alex Van der Eb in Leiden, Netherlands. The use of 293 refers to the experiment number referenced in his laboratory note book.

Here we present a genomic, transcriptomic and metabolic gene analysis of six of the most widely used HEK293 cell lines. Changes in gene copy and expression between industrial progeny cell lines and the original HEK293 were associated with cellular component organization, cell motility and cell adhesion.

HEK293 cells are available from ATCC (www.atcc.org) , catalog# CRL-1573. It is easy to grow and transfect and have been widely used for cell biology research and also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy. Materials: 15ml/High Clarity Polypropylene Conical tubes(BD Falcon Cat.# REF 352096)