Contig or scaffold N50 is a weighted median statistic such that 50% of the entire assembly is contained in contigs or scaffolds equal to or larger than this value. Mathematically: Given a set of sequences of varying lengths, the N50 length is defined as the length N for which 50% of all bases in the sequences are in a sequence of length L < N.

Jul 3, 2021 · $\begingroup$ @user438383 Because my professors always told me only truly understand something if you can explain it to your 5 year old brother :) And also because I thought this is a positive and supportive place where people are encouraged to ask questions that other people are encouraged to answer (collecting votes I assume).

May 19, 2015 · Can someone explain the differences between sequence, reads, and contigs of genetic material such as DNA, if possible with an example? I am new to bioinformatics, and I have not found any conclusive

Jul 18, 2012 · Unequivocally, YES. Before DNA sequencing was used to deduce the sequence of cloned or reverse-transcribed RNA molecules, slower and more cumbersome direct sequencing of RNA was performed using the method of Sanger et al. (Sanger, F., Brownlee, G. G. and Barrel B. G. (1965) J. Mol. Biol. 13, pp. 373–398).

Jul 27, 2017 · pycoQC: N50 is zero (or null). overflow encountered in scalar add Why did C99 have to add the underscored keywords for _Bool _Complex _Imaginary but not for inline or restrict? How does the Ranger feature Favored Enemy (with Beasts chosen as the creature type) work with an unknown beast?

Jun 1, 2017 · I am trying to understand the difference between read based and contig based alignment. Is contig based alignment refer to de novo assembly and then it is align to a reference genome.

Jun 24, 2015 · Stack Exchange Network. Stack Exchange network consists of 183 Q&A communities including Stack Overflow, the largest, most trusted online community for developers to learn, share their knowledge, and build their careers.

May 16, 2017 · From what I understand the 'Great Oxidation Event' occurred around 2.4 billion - 2.3 billion years ago, when cyanobacteria flooded Earth’s atmosphere with oxygen. The 'boring billion' period was ...

When talking about a maternal unbroken linage and a paternal unbroken linage, is the meaning that Mitochondrial Eve had a linage of only women reaching every person today, and the same with Y-chromosomal Adam and men?

Aug 28, 2017 · pycoQC: N50 is zero (or null). overflow encountered in scalar add Is it problematic to compare 2 nutritional interventions with different duration Ubuntu 24.04.1: "Oh no! something has gone wrong" after some EFI driver stuff