DNA purified from PCR reaction mix was phosphorylated and inserted into dephosphorylated pEGFP-C1 and pEGFP-N1 vectors. Plasmids were transformed to 5-alpha competent E. coli cells (NEB) and ...
Furin is identified as the key enzyme responsible for cleaving the apelin precursor. Inhibiting this cleavage could serve as ...
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