Microglia were isolated using density gradient centrifugation after brain tissue digestion and filtration. Dietary preferences were tested by offering mice high-fat or high-sugar diets alongside ...

Abstract Research of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) and Fibromyalgia (FM), two acquired chronic illnesses affecting mainly females, has failed to ascertain their frequent ...

Mononuclear cells (MNCs) were isolated from fresh bone marrow (BM) by Ficoll density gradient centrifugation. To do so, BM was diluted 1:2 with phosphate-buffered saline (PBS) and loaded on top of ...

Cell culture supernatant (SN) was obtained by centrifugation. PD-1 ... We separated peripheral blood mononuclear cells (PBMC) by Ficoll-Paque density gradient.21 We cultured PBMC for 48 hours at a ...

2 g dry sediment samples were extracted by 15 ml EDTA buffer solution and 15 ml acetonitrile; after oscillation (10 min, 200 rpm), ultrasonication (15 min), centrifugation ... 5 μL; and gradient ...

Induces potent and long-lasting immune responses to defined antigens or whole pathogens (particles), mainly through the induction of high-affinity antibodies derived from GCs and produced by ...

The management of Leishmania donova ni (LD), responsible for fatal visceral leishmaniasis (VL), faces increasing challenges due to rising drug-unresponsiveness, leading to increasing treatment ...

State Key Laboratory of Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University ...

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