Department of Chemistry, Faculty of Arts and Sciences, Center for Advanced Materials Research, Beijing Normal University, Zhuhai 519087, China Key Laboratory of Theoretical and Computational ...

For cell survival experiments, AAV plasmids were transfected with empty GFP plasmid in a 1:1 ratio, sorted using BD FACSMelody TM Cell sorter and replate for 3 days. Cells were then analysed using ...

In this study, we used three plasmid packaging systems, including the plenti-GIII-CMV-CBH-GFP-2A-Puro-CSFV NS5A transfer plasmid, the pSPAX2 packaging plasmid, and the envelope plasmid pMD2G. HEK-293T ...

School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, P.R. China School of Chemical & Material Engineering, Jiangnan University, Wuxi 214122, P.R. China ...

Plasmid DNA is an essential part of cell and gene therapy products. It is utilized as a template vector for cell therapy and non-viral gene therapy and as a raw material to produce adeno-associated ...

Fluctuation ('noise') in gene expression is critical for mammalian cellular processes. Numerous mechanisms contribute to its origins, yet the mechanisms behind large fluctuations that are induced by ...

Additionally, HCECs were transfected with MICA plasmid or treated with IFN-γ and NKG2D-mAb and ... Differential miRNA expression, miRNA4448 expression after pLenti-CMV-GFP-miR4448 transfection, and ...