All antibodies were purchased from BioLegend, BD Biosciences, or ThermoFisher Scientific. An unconjugated anti-CD16/CD32 antibody (clone 2.4G2, UCSF Antibody Core) was used to block non-specific ...
Liver was perfused through the vena cava with 10 ml ice-cold PBS. During perfusion, the hepatic portal vein was cut to allow outflow of the blood from the liver. Afterwards, liver was gently meshed ...
Over half of breast-cancer-related deaths are due to recurrence 5 or more years after initial diagnosis and treatment. This latency suggests that a population of residual tumor cells can survive ...
Following standard protocols, cells were stained with anti-CD4-FITC, anti-CD3e-PE, anti-T-bet-APC, and anti-Gata3-PerCP as well as with anti-CD4-FITC, anti-CD3e-PerCP, and anti-Foxp3-PE, both ...
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