This extension of real-time PCR technology, called reverse transcription PCR (RT-PCR), combines real-time PCR with reverse transcription, the process that makes DNA from mRNA. RT-PCR can be used ...

In order for a virus like the COVID-19 virus to be detected early in the body using real time RT–PCR, scientists need to convert the RNA to DNA. This is a process called ‘reverse transcription’. They ...

For household contacts of individuals with COVID-19, taking ensitrelvir within 72 hours of symptoms is effective at ...

The Quantiplus MTB FAST Detection Kit, developed by Huwel Lifesciences, has been approved by Indian Council of Medical ...

Since the emergence of COVID-19 in late 2019, the gold standard in testing for the disease has been a nuclear-derived technique: real-time reverse transcription–polymerase chain reaction, or real-time ...

Despite variations by age group and influenza virus type, the 2023-2024 influenza vaccine was protective against outpatient illness in pediatric and adult patients.

Quantitative polymerase chain reaction (qPCR) is a molecular technique that has reshaped ... 70-200 bp). Design forward and reverse primers with similar melting temperatures (Tm), aiming for a Tm of ...

PCR-Machines are classified depending on the capacity of PCR used such as Nested PCR or Reverse Transcriptase PCR (RT-PCR ), or Multiplex RT PCR. PCR-machines are used for replication of DNA ...

This method facilitates sequencing multiple regions of interest at the same time. Several components are involved in a PCR including template DNA, forward and reverse primers, DNA polymerase enzyme, ...