The scientists successfully adapted it to a variety of AAV serotypes and genes of interest, including AAV8-GFP ... and protein depletion, as well as yields during each step of the purification ...

After oligonucleotide annealing and extension, the PCR-purified (PCR purification kit; QIAGEN ... and 16.67 ng/well pMAX-GFP plasmid. With these methods, cells were typically transfected at ...

Purification and analysis of human T cells ... In some experiments, a plasmid encoding green fluorescent protein (GFP, 1μg) was transfected into RIP-deficient Jurkat T cells along with the ...

PCR products were purified by Qiaquick PCR purification. Samples were sequenced on HiSeq ... cells will therefore initially yield phosphorylated protein, and this form of GFP-CLASP2 is not able to ...

Nuclera’s Key Account Manager shares how their eProtein Discovery system is propelling rapid recombinant protein optimization and its utilization in drug discovery.

The use of electrical impulses also speeds up the purification process, Ferrand-Drake del Castillo says, explaining that “instant millisecond switches reduce the cycle time and use of raw materials by ...

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Maybe you are not consuming enough protein. This essential nutrient is essential for muscle growth, recovery, and tissue repair, making it just as important as your workouts. Without enough of it ...

Protein is kind of the It Girl of nutrients right now. It's the macro that's good at everything: building muscle, supporting immune function, sustaining energy levels, shoring up bone health, and ...

A temporally resolved CRISPR-Cas9 screen reveals DAC-induced DNA damage drives trans-cell cycle cytotoxicity that depends on ...

For anyone looking to increase their protein intake, high-protein nuts are a top choice. Snack on them by the handful, add them as a garnish sprinkled on soups and salads, or incorporate them into ...