Bound ligand was measured by scintillation counting. The pCSUACG plasmid (U6-shRNA-AR-CMV-GFP) was constructed by ligating the Bam HI/ Eco RI digests of pCSCG and the U6-shRNA-AR PCR product.

The plasmid DNA vaccine (above) carries the genetic code for a piece of pathogen or tumor antigen. The plasmid vector is taken up into cells and transcribed in the nucleus (1).

Lanes 4–6, Cells cotransfected with 1.0 μg substrate plasmid and 2.0 μg, 0.2 μg, or 0.02 μg of CMV-Cre-hGH expression plasmid, respectively. The similar yield of recombined product in all three lanes ...

To silence BCAS2 expression, shRNA constructs in pLL 3.7-GFP plasmid were generated to target the following sequences: shRNA1, GAATGTGTAAACAATTCTA; shRNA2: GAAGGAACTTCAGAAGTTA. Transfection was ...

Figure 1: Correlations between polysome and nucleoid dynamics at the single-cell level. CJW7323 cells were grown in M9gluCAAT in a microfluidic device. A. Schematic illustrating observable nucleoid ...

Abstract—Fibroblast growth factor (FGF)-1 plays important roles during myocardial and coronary morphogenesis. FGF-1 is also involved in the physiological response of the adult heart against ischemia, ...

Coumarin derivatives have found application as probes for the hydroxyl radical because one of the products of the reaction between them is a highly fluorescent umbelliferone. We have examined the ...

B) Representative scheme generating the OG:A-containing or OG analog:A-containing GFP plasmid reporter. The plasmid is first nicked with the nickase Nb.Bpu10i. Ligation of the OG-containing ...

Abstract Background— Vascular endothelial growth factor-A (VEGF-A) promotes angiogenesis but causes adverse side effects such as edema or tissue inflammation. VEGF-E, found in the genome of the Orf ...