Here, we use multiple PCR-based targeted next-generation sequencing (tNGS ... Library concentrations were quantified using a Qubit 4.0 fluorometer (Invitrogen), requiring that the amount of data that ...
PCR-based techniques are methods that rely on the polymerase chain reaction (PCR) to amplify stretches of DNA by creating many identical or near-identical copies. For example, PCR amplification ...
The PCR product was diluted 1/200 and used for a second PCR run ... The DNA concentration of each sample was measured with Qubit 2.0 Fluorometer (Thermo Fisher Scientific) and 250 ng of each sample ...
The Applied Biosystems® StepOneâ„¢ Real-Time PCR Systems offer intuitive software, Fast (<40 min) and standard (<2 hr) runs, and are configured for PC-free or networked operation. These factory ...
The OpenArray® Real-Time PCR Platform puts the control in the hands to get the answers you need. It delivers the best combination of convenience, flexibility, throughput, and cost-effectiveness ...