The multiplex PCR was done with odd-numbered primer pairs (S201 ... United States) and Quantus™ Fluorometer, according to the manufacturer’s instructions. The sequencing libraries for iSeq100 were ...

Here, we use multiple PCR-based targeted next-generation sequencing (tNGS ... Library concentrations were quantified using a Qubit 4.0 fluorometer (Invitrogen), requiring that the amount of data that ...

The E-Gel ® electrophoresis system is ideal for routine analysis of PCR products, restriction digests, and plasmid preparations. No gels to pour, no buffer to make, no staining/destaining ...

DNA extracted using the PureFood Kit can be used directly in a variety of downstream applications such as real-time PCR, gel electrophoresis, microarrays, and sequencing (e.g., NGS and Sanger).

This is an important and solid study that examines the role of TFAM, a protein that helps maintain mtDNA, in mtDNA mutator mice. The authors have demonstrated that TFAM's counteractive role in mtDNA ...