Hoechst stain was used to locate cells within the tendon ... suggesting a non-linear time-dependent and dose-dependent uptake pattern. N = 3. C. Flow cytometry imaging of iTTFs incubated with 5 µg/mL ...

The cells were resuspended in Annexin V APC/DAPI staining solution and incubated in the ... To test this, we quantified the macrophage subtypes using flow cytometry after staining the cell suspensions ...

Surface expression of MmMYL3 was analyzed by flow cytometry. (G and H ... were detected by immunofluorescence staining. Cell nuclei were stained with DAPI. Bar = 10 μm. Data were collected from three ...

Nuclei were detected in the DAPI channel using a minimum cross-entropy segmentation algorithm. Nuclei outlines were expanded by 15 pixels to partially cover the cytoplasm of the cells. In this region, ...