The fungal strain was cultured and harvested on cellophane membranes overlaid on the surface of PDA plates for 7 days. The mycelia were homogenized in liquid nitrogen, and the dsRNA was isolated using ...

The concentrations of the siRNA and miRNA used in transfection was 5 pmol/well for 96-well-plate ... RNA extraction and real-time RT-PCR analysis were performed as previously described (29). Briefly, ...

A temporally resolved CRISPR-Cas9 screen reveals DAC-induced DNA damage drives trans-cell cycle cytotoxicity that depends on ...

Aneuploidy is frequent in cancer, but in vitro it triggers multiple cellular stresses and often delays proliferation. Through ...

This is an important and solid study that examines the role of TFAM, a protein that helps maintain mtDNA, in mtDNA mutator mice. The authors have demonstrated that TFAM's counteractive role in mtDNA ...

The management of Leishmania donova ni (LD), responsible for fatal visceral leishmaniasis (VL), faces increasing challenges due to rising drug-unresponsiveness, leading to increasing treatment ...

into devices the size of a microscope slide. The UF team has developed a microfluidic device for DNA purification that could speed up analysis of laboratory samples and match or exceed the purity ...

The redness, inflammation, and pain of sunburn may stem from damage to RNA, not DNA. “Sunburn damages the DNA, leading to cell death and inflammation. So the textbooks say,” said Assistant ...

Recent research reveals that DNA and RNA epigenetics, previously studied as independent systems, actually function as a complementary regulatory network. This dual mechanism enables precise gene ...