To complete our studies, we substituted emerald GFP (emGFP) for YFP. Using two-photon excitation, CFP can be excited at 800–825 nm with little direct excitation of emGFP (which has two-photon ...

GFP can be analysed with YFP on flow cytometers with appropriate filter and mirror changes. The GFP detector on a BD instrument should have a 502LP mirror with a 510/10nm BP filter. While the PE ...