In our studies with COS cells expressing CFP-tagged EGF receptor and YFP-tagged mutant PTP1B (D181A,Q262A), we observed large increases in what we thought was CFP fluorescence when the YFP was ...

Figure 1: ATP concentration affects signal efficacy of CFP-YFP–based FRET sensors. It is important to note further that the ratio changes (8–45% change in YFP/CFP ratio) are within the range ...