The method described here 1 uses carrier ampholytes to form a pH gradient in a long, thin (1.2-mm) focusing gel composed of a low percentage (2.7%) of acrylamide and containing 9.5 M urea and 2% ...

Buffer D is 20 mM HEPES-KOH (pH 7.9), 20% glycerol (vol/vol), 0.2 mM EDTA ... 4| Load the samples directly (with no dye) onto the gel. Carefully layer the mix onto the bottom of the well and ...