Note, the pH is 9.2 (the optimal for long PCR) without adjustment; some vectors may require pH 7.2. 10-40 dNTP mix: 10 mM each dNTP, 40 mM MgCl 2; dissolve the dry form of each dNTP in water to ...

DNA polymerases play a central role in maintenance of genetic information, and the structures of polymerases in complex with DNA and dNTP provide valuable insights into mechanisms utilized by DNA ...