4| To the cDNA in a volume of 10 μl add ... by 20–100 nt as measured by electrophoresis through a 1% neutral agarose gel. 7| Withdraw a sample (5–10 μl) from each amplification reaction ...

We used human heart, placenta and testis total RNA (Clontech) and human brain poly(A) + RNA (Clontech) for cDNA synthesis using ... We ran RT–PCR products on a 1% agarose gel and visualized ...