To complete our studies, we substituted emerald GFP (emGFP) for YFP. Using two-photon excitation, CFP can be excited at 800–825 nm with little direct excitation of emGFP (which has two-photon ...

GFP can be analysed with YFP on flow cytometers with appropriate filter and mirror changes. The GFP detector on a BD instrument should have a 502LP mirror with a 510/10nm BP filter. While the PE ...

3) thus preventing discrimination of CFP and photobleached YFP on the basis of their emission or excitation spectra. During photobleaching FRET experiments, any increase in the CFP channel could ...