Unfortunately, FRET pairs incorporating orange, red or far-red fluorescent proteins have not offered performance on par with the traditional CFP-YFP pair. A common problem when using red-shifted ...

and yellow fluorescent protein (YFP) 2. Figure 1: ATP concentration affects signal efficacy of CFP-YFP–based FRET sensors. It is important to note further that the ratio changes (8–45% change ...

The emission of YFP peaks at 530nm, whilst CFP emits maximally at 475 and 553nm respectively. Conventional flow cytometers equipped with argon and violet lasers will thus excite YFP well but ...