To complete our studies, we substituted emerald GFP (emGFP) for YFP. Using two-photon excitation, CFP can be excited at 800–825 nm with little direct excitation of emGFP (which has two-photon ...

3) thus preventing discrimination of CFP and photobleached YFP on the basis of their emission or excitation spectra. During photobleaching FRET experiments, any increase in the CFP channel could ...

The emission of YFP peaks at 530nm, whilst CFP emits maximally at 475 and 553nm respectively ... emission collected in the first fluorescence parameter at 575nm, see figure. GFP can be analysed with ...