In our studies with COS cells expressing CFP-tagged EGF receptor and YFP-tagged mutant PTP1B (D181A,Q262A), we observed large increases in what we thought was CFP fluorescence when the YFP was ...

You have full access to this article via your institution. There are several methods for removing MAPs from MTP to produce purified tubulin: using DEAE-Sephadex or phosphocellulose chromatography ...

The role of centrosomal tubulin glutamylation has remained elusive for decades. Here, a new tool enables precise spatiotemporal control of this modification, showing that it physically recruits ...