Currently, housekeeping proteins (HKPs) like actin, tubulin, or GAPDH are often used to check for equal loading or to compensate potential loading differences. However, these loading controls have ...

The microtubule-associated protein XMAP215 promotes microtubule growth, but how this occurs has been unclear. To address this question, Gary Brouhard et al. developed a single-molecule ...

You have full access to this article via your institution. There are several methods for removing MAPs from MTP to produce purified tubulin: using DEAE-Sephadex or phosphocellulose chromatography ...