Users can search the database by gene, species or type of assay (TaqMan, SYBR Green I or RT-PCR) to find the primer's location, sequence and original reference. Similarly, the RTPrimerDB database ...

In his commentary, W.P. Halford (Nat. Biotechnol. 17, 835, 1999) proposes that most quantitative PCR applications require neither the use of internal standards (competitive PCR), nor a ...

Full compatibility with any standard or fast-cycling 384- or 96-well plates and TaqMan® Array Micro Fluidic Cards and Reagents makes high productivity real-time PCR accessible to labs of any size.

Although the SYBR Green I-based real-time PCR assay is less specific than the TaqMan real-time RT-PCR, it does have certain advantages, such as reduced expense (especially for high throughput ...

This enables real-time PCR in automated environments for increased ... qPCR Increase efficiency with TaqMan Array cards on the QuantStudio 7 Pro system. Load up to 384 targets with just 8 ...

In these cases the detection of HAV RNA by RT-PCR or real-time PCR can clarify the diagnosis of acute hepatitis A in patients without anti-HAV IgM. [64] High and persistent excretion of HAV was ...

reagents for cDNA synthesis and either TaqMan or SYBR Green master mixes for real-time PCR analysis. Extraordinary ease and speed—96 samples for qRT-PCR in typically <10 min No tedious RNA ...