A method to select for proteins that are highly efficient at folding yields 'superfolder' GFP, an optimized GFP for use in protein fusions. You have full access to this article via your institution.

By genotyping thousands of Arabidopsis plants, they found that using the RPS5A promotor to express Cas9 led to the highest mutation rate, and that flanking the Cas9 protein with bipartite NLS was ...