PCR genetic analysis has been in the spotlight since COVID-19, but light is now further facilitating PCR-free methods. Osaka ...

Light accelerates DNA hybridization using gold nanoparticles and polystyrene microparticles as probes for PCR-free DNA detection.

There are many popular chemistries for real-time PCR. One class uses different fluorescent dyes incorporated in short oligonucleotide probes specific for the amplified target. The second class ...

ProbeLibrary, a fast, specific and flexible format for quantitative real-time PCR, is described. The ProbeLibrary concept is based on the fact that just 90 short probes provide transcriptome-wide ...

Multiplex real-time PCR-based diagnostic tests facilitate the simultaneous detection of multiple pathogens in a single assay, ...

is an extension of traditional PCR, where DNA amplification over time is quantitated by measuring fluorescence from dyes intercalating into nascent DNA strands or by cleavage of target-specific probes ...