The team observed a 4.4-fold increase in the editing efficiency of this modified nuclease, surpassing most of the commonly used RNA-guided endonucleases, including CjCas9. They called this variant ...

Mitochondrial diseases, arising from mutations in mitochondrial DNA (mtDNA), are a diverse group of disorders that lead to ...

The complex adopts a more compact structure when bound to double-stranded DNA (dsDNA), with the target DNA strand making a pronounced U-turn and interacting with the HNH nuclease domain.

1| Pellet ∼10 8 cells at 300g at 4 °C for 10 min in a centrifuge. The nuclei should be kept cold throughout the procedure. Manipulations and transport to the centrifuge should be done on ice. 2 ...

Rapidly add 300 μl of ice-cold nuclease S1 digestion buffer and immediately remove the tube from the water bath. Vortex gently to mix and then transfer the tube to a water bath set at the ...

This nonexclusive sublicense grants Vita Therapeutics access to the engineered hfCas12Max nuclease and optimized hfCas12Max guide RNAs for use in preclinical and clinical applications, including in ...

To uncover the mechanisms for nuclease regulation, we apply a multidisciplinary approach that includes hybrid structural biology, biochemistry, and cell biology. Together our work aims to understand ...