DNA ligases catalyze the joining of a 5’-phosphate-terminated strand to a 3’-hydroxyl-terminated strand. Ligation depends on magnesium and a high-energy cofactor, either ATP or NAD +. The reaction ...
Figure 2: Ligation of circularizable probes by Ampligase, a thermostable DNA ligase. To facilitate allele discrimination in genomic DNA, we designed a second oligonucleotide primer to bind ...
ATP-dependent RNA ligases join 3’-OH and 5’-PO 4 RNA termini via three nucleotidyl transfer steps similar to those of DNA ligases: (Step 1) ligase reacts with ATP to form a covalent ...
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