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The pH of the solution was controlled using three different bases, which included tetramethylammonium hydroxide (TMAOH), sodium hydroxide (NaOH), and potassium hydroxide (KOH). The precipitated ...
Both para-nitrophenylphosphate and p-nitrophenol are colorless at acidic pH values. Addition of 0.05 M NaOH after 30 min incubation at 37 °C will stop the reaction due to an increase in the pH of the ...