Embedding and polymerization are done at low temperatures (-30 - -55° C). HPF followed by freeze-substitution with 0.5% uranyl acetate in methanol results in excellent preservation of actin-filaments ...

Image 1 Cell monolayers can be sectioned parallel or perpendicular to the coverslip. NRK-52E cells were either detached from the substratum and processed as cell pellet (A) or grown on glass coverslip ...